Plants Use RNA to Talk to Neighbors. TheScientist

 

 https://www.the-scientist.com/news-opinion/plants-use-rna-to-talk-to-neighbors-69337

 Fra TheScientist

Plants Use RNA to Talk to Neighbors

A study finds that plants sharing the same growth medium can exchange microRNAs that silence genes in the recipient, suggesting the nucleic acids may act as signaling molecules.

alejandra manjarrez
Alejandra Manjarrez
alejandra manjarrez

Oct 21, 2021

89

ABOVE: Arabidopsis thaliana flowers
PEGGY GREB, USDA ARS

Plants use a variety of mechanisms to communicate with other organisms, including one another. Volatile compounds can signal flowering and attract pollinators, for instance, and mycorrhizal fungal networks can transmit warnings or transfer resources. Small RNAs are on that list of communication molecules, and new findings confirm their potential: according to a paper published October 14 in Nature Plants, the plant Arabidopsis thaliana secretes microRNAs (miRNAs)—a type of small, single-stranded RNAs—into its liquid growth medium. Nearby individuals then take up these RNAs, which alter their gene expression patterns by binding to messenger RNAs and preventing certain genes from being translated into proteins (a process known as RNA interference).

Hailing Jin, a plant molecular geneticist at the University of California, Riverside, who was not involved in the study, says it’s exciting to see that plants can take up microRNAs from the environment, including those “secreted by other plants through the roots.”

That small RNAs can be exchanged between different organisms is not new. In addition to their role as regulators of gene expression within an individual—as part of development or in response to stress—they have been implicated in defense against pathogens in recent years. For instance, Arabidopsis cells infected with the pathogenic fungus Botrytis cinerea secrete small RNAs packed in extracellular vesicles that, when delivered into their attacker, inhibit its virulence. Plants are also able to take up sprayed RNA molecules targeting genes from pathogens. The recent findings are the first evidence of plants taking up RNA secreted by other plants into the environment.

See “RNA Interference Between Kingdoms

“The results were totally unexpected,” Pierdomenico Perata, a plant physiologist at the Sant’Anna School of Advanced Studies in Pisa, Italy, and coauthor on the study, writes in an email to The Scientist. Given RNAs’ reputation as “highly unstable” molecules outside of a cell, he writes his team “expected miRNA to be incompatible with a non-sterile environment such as the growth medium.”

Perata relates that his team was working “on a totally unrelated topic”—exploring the role of RNA interference under limited oxygen availability—and it was for that purpose that they hydroponically grew Arabidopsis plants engineered to produce large quantities of specific miRNAs. As they simply wanted them to produce seeds, he adds, the researchers “didn’t care about placing different plant lines in separate trays.” But then they noticed that wildtype plants sharing the mutants’ hydroponic solution had phenotypes different from those expected—for example, those growing next to mutants that overexpressed miRNAs targeting developmental genes had their own flowering time altered. According to Perata, that’s when he and his colleagues wondered “if miRNAs could be released in the liquid growth medium, thereby affecting the phenotype of wildtype plants.”

The researchers tested the hydroponic solution, and lo and behold, they detected miRNAs. These miRNAs were present regardless of whether the plants growing in the solution were wildtype or mutated to overexpress them, although more RNAs were detected in the mutants’ solution. Furthermore, cultivating both lines in the same solution resulted in wildtype plants with notably lower expression levels of the genes targeted by the mutants’ boosted miRNA molecules. Applying miRNAs extracted from the mutants or chemically synthesized equivalents also reduced gene expression.

Why would a plant need to affect another plant’s gene expression? One possibility, Perata posits, is that “sharing information by exchanging RNA would allow plants experiencing a stress to warn nearby plants, not yet affected by the stress.” Competition could be another explanation, he writes; for instance, if a plant releasing miRNAs “could inhibit physiological functions in a nearby plant,” it could gain “a competitive advantage for the use of resources.”

One unanswered question is how the plants take up these tiny molecules from the environment. Previous work studying RNA exchange between plants and pathogens suggests that exosomes, a type of vesicles that can act as delivery vehicles, might be involved in the process. However, the researchers found that applying extracted, presumably naked miRNAs or synthetic RNAs had an effect in gene expression, suggesting that exosomes aren’t needed for uptake.

Hui-Shan Guo, a plant microbiologist at the Institute of Microbiology at the Chinese Academy of Sciences, says the study’s evidence for naked RNA uptake confirms previous reports of gene silencing via sprayed-on RNA. She suggests in an email to The Scientist that, as with nutrients, plants might actively assimilate small RNAs from the environment. But unlike the substances plants are known to import, naked RNA molecules “were thought instable,” she says, so “RNA uptake was ignored or underestimated.”

See “Exosomes Make Their Debut in Plant Research

Jin agrees that the evidence in the paper supports the hypothesis that plants can uptake naked miRNA, but she says she wonders whether their secretion still occurs via exosomes from roots—a question the authors did not explore. She adds she also suspects that these vesicles could protect the miRNAs, helping the plants to accomplish a more efficient uptake. Otherwise, the molecules could be more easily degraded in the soil and in the environment, she speculates.

Guo points out that, as this mechanism has only been explored in hydroponically grown plants, it’s not yet clear “whether seedlings growing in soil . . . would have effects on regulation of gene expression in [nearby] plants”—something future studies could examine.

Jin adds that these new findings open a lot of new questions, and that there is likely much more to learn about the role of RNA in plant communication. What we currently know about it is just the “tip of the iceberg,” she concludes.

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  236. 民意調查是什麼?民調什麼意思?
    民意調查又稱為輿論調查或民意測驗,簡稱民調。一般而言,民調是一種為了解公眾對某些政治、社會問題與政策的意見和態度,由專業民調公司或媒體進行的調查方法。

    目的在於通過網路、電話、或書面等媒介,對大量樣本的問卷調查抽樣,利用統計學的抽樣理論來推斷較為客觀,且能較為精確地推論社會輿論或民意動向的一種方法。
    以下是民意調查的一些基本特點和重要性:

    抽樣:由於不可能向每一個人詢問意見,所以調查者會選擇一個代表性的樣本進行調查。這樣本的大小和抽樣方法都會影響調查的準確性和可靠性。
    問卷設計:為了確保獲得可靠的結果,問卷必須經過精心設計,問題要清晰、不帶偏見,且易於理解。
    數據分析:收集到的數據將被分析以得出結論。這可能包括計算百分比、平均值、標準差等,以及更複雜的統計分析。
    多種用途:民意調查可以用於各種目的,包括政策制定、選舉預測、市場研究、社會科學研究等。
    限制:雖然民意調查是一個有價值的工具,但它也有其限制。例如,樣本可能不完全代表目標人群,或者問卷的設計可能導致偏見。
    影響決策:民意調查的結果常常被政府、企業和其他組織用來影響其決策。
    透明度和誠實:為了維護調查的可信度,調查組織應該提供其調查方法、樣本大小、抽樣方法和可能的誤差範圍等詳細資訊。
    民調是怎麼調查的?
    民意調查(輿論調查)的意義是指為瞭解大多數民眾的看法、意見、利益與需求,以科學、系統與公正的資料,蒐集可以代表全部群眾(母體)的部分群眾(抽樣),設計問卷題目後,以人工或電腦詢問部分民眾對特定議題的看法與評價,利用抽樣出來部分民眾的意見與看法,來推論目前全部民眾的意見與看法,藉以衡量社會與政治的狀態。

    以下是進行民調調查的基本步驟:

    定義目標和目的:首先,調查者需要明確調查的目的。是要了解公眾對某個政策的看法?還是要評估某個政治候選人的支持率?
    設計問卷:根據調查目的,研究者會設計一份問卷。問卷應該包含清晰、不帶偏見的問題,並避免導向性的語言。
    選擇樣本:因為通常不可能調查所有人,所以會選擇一部分人作為代表。這部分人被稱為“樣本”。最理想的情況是使用隨機抽樣,以確保每個人都有被選中的機會。
    收集數據:有多種方法可以收集數據,如面對面訪問、電話訪問、郵件調查或在線調查。
    數據分析:一旦數據被收集,研究者會使用統計工具和技術進行分析,得出結論或洞見。
    報告結果:分析完數據後,研究者會編寫報告或發布結果。報告通常會提供調查方法、樣本大小、誤差範圍和主要發現。
    解釋誤差範圍:多數民調報告都會提供誤差範圍,例如“±3%”。這表示實際的結果有可能在報告結果的3%範圍內上下浮動。
    民調調查的質量和可信度很大程度上取決於其設計和實施的方法。若是由專業和無偏見的組織進行,且使用科學的方法,那麼民調結果往往較為可靠。但即使是最高質量的民調也會有一定的誤差,因此解讀時應保持批判性思考。

    為什麼要做民調?
    民調提供了一種系統性的方式來了解大眾的意見、態度和信念。進行民調的原因多種多樣,以下是一些主要的動機:

    政策制定和評估:政府和政策制定者進行民調,以了解公眾對某一議題或政策的看法。這有助於制定或調整政策,以反映大眾的需求和意見。
    選舉和政治活動:政黨和候選人通常使用民調來評估自己在選舉中的地位,了解哪些議題對選民最重要,以及如何調整策略以吸引更多支持。
    市場研究:企業和組織進行民調以了解消費者對產品、服務或品牌的態度,從而制定或調整市場策略。
    社會科學研究:學者和研究者使用民調來了解人們的社會、文化和心理特征,以及其與行為的關係。
    公眾與媒體的期望:民調提供了一種方式,使公眾、政府和企業得以了解社會的整體趨勢和態度。媒體也經常報導民調結果,提供公眾對當前議題的見解。
    提供反饋和評估:無論是企業還是政府,都可以透過民調了解其表現、服務或政策的效果,並根據反饋進行改進。
    預測和趨勢分析:民調可以幫助預測某些趨勢或行為的未來發展,如選舉結果、市場需求等。
    教育和提高公眾意識:通過進行和公布民調,可以促使公眾對某一議題或問題有更深入的了解和討論。

    民調可信嗎?
    民意調查的結果數據隨處可見,尤其是政治性民調結果幾乎可說是天天在新聞上放送,對總統的滿意度下降了多少百分比,然而大家又信多少?

    在景美市場的訪問中,我們了解到民眾對民調有一些普遍的觀點。大多數受訪者表示,他們對民調的可信度存有疑慮,主要原因是他們擔心政府可能會在調查中進行操控,以符合特定政治目標。

    受訪者還提到,民意調查的結果通常不會對他們的投票意願產生影響。換句話說,他們的選擇通常受到更多因素的影響,例如候選人的政策立場和政府做事的認真與否,而不是單純依賴民調結果。

    從訪問中我們可以得出的結論是,大多數民眾對民調持謹慎態度,並認為它們對他們的投票決策影響有限。

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  246. 民意調查是什麼?民調什麼意思?
    民意調查又稱為輿論調查或民意測驗,簡稱民調。一般而言,民調是一種為了解公眾對某些政治、社會問題與政策的意見和態度,由專業民調公司或媒體進行的調查方法。

    目的在於通過網路、電話、或書面等媒介,對大量樣本的問卷調查抽樣,利用統計學的抽樣理論來推斷較為客觀,且能較為精確地推論社會輿論或民意動向的一種方法。
    以下是民意調查的一些基本特點和重要性:

    抽樣:由於不可能向每一個人詢問意見,所以調查者會選擇一個代表性的樣本進行調查。這樣本的大小和抽樣方法都會影響調查的準確性和可靠性。
    問卷設計:為了確保獲得可靠的結果,問卷必須經過精心設計,問題要清晰、不帶偏見,且易於理解。
    數據分析:收集到的數據將被分析以得出結論。這可能包括計算百分比、平均值、標準差等,以及更複雜的統計分析。
    多種用途:民意調查可以用於各種目的,包括政策制定、選舉預測、市場研究、社會科學研究等。
    限制:雖然民意調查是一個有價值的工具,但它也有其限制。例如,樣本可能不完全代表目標人群,或者問卷的設計可能導致偏見。
    影響決策:民意調查的結果常常被政府、企業和其他組織用來影響其決策。
    透明度和誠實:為了維護調查的可信度,調查組織應該提供其調查方法、樣本大小、抽樣方法和可能的誤差範圍等詳細資訊。
    民調是怎麼調查的?
    民意調查(輿論調查)的意義是指為瞭解大多數民眾的看法、意見、利益與需求,以科學、系統與公正的資料,蒐集可以代表全部群眾(母體)的部分群眾(抽樣),設計問卷題目後,以人工或電腦詢問部分民眾對特定議題的看法與評價,利用抽樣出來部分民眾的意見與看法,來推論目前全部民眾的意見與看法,藉以衡量社會與政治的狀態。

    以下是進行民調調查的基本步驟:

    定義目標和目的:首先,調查者需要明確調查的目的。是要了解公眾對某個政策的看法?還是要評估某個政治候選人的支持率?
    設計問卷:根據調查目的,研究者會設計一份問卷。問卷應該包含清晰、不帶偏見的問題,並避免導向性的語言。
    選擇樣本:因為通常不可能調查所有人,所以會選擇一部分人作為代表。這部分人被稱為“樣本”。最理想的情況是使用隨機抽樣,以確保每個人都有被選中的機會。
    收集數據:有多種方法可以收集數據,如面對面訪問、電話訪問、郵件調查或在線調查。
    數據分析:一旦數據被收集,研究者會使用統計工具和技術進行分析,得出結論或洞見。
    報告結果:分析完數據後,研究者會編寫報告或發布結果。報告通常會提供調查方法、樣本大小、誤差範圍和主要發現。
    解釋誤差範圍:多數民調報告都會提供誤差範圍,例如“±3%”。這表示實際的結果有可能在報告結果的3%範圍內上下浮動。
    民調調查的質量和可信度很大程度上取決於其設計和實施的方法。若是由專業和無偏見的組織進行,且使用科學的方法,那麼民調結果往往較為可靠。但即使是最高質量的民調也會有一定的誤差,因此解讀時應保持批判性思考。

    為什麼要做民調?
    民調提供了一種系統性的方式來了解大眾的意見、態度和信念。進行民調的原因多種多樣,以下是一些主要的動機:

    政策制定和評估:政府和政策制定者進行民調,以了解公眾對某一議題或政策的看法。這有助於制定或調整政策,以反映大眾的需求和意見。
    選舉和政治活動:政黨和候選人通常使用民調來評估自己在選舉中的地位,了解哪些議題對選民最重要,以及如何調整策略以吸引更多支持。
    市場研究:企業和組織進行民調以了解消費者對產品、服務或品牌的態度,從而制定或調整市場策略。
    社會科學研究:學者和研究者使用民調來了解人們的社會、文化和心理特征,以及其與行為的關係。
    公眾與媒體的期望:民調提供了一種方式,使公眾、政府和企業得以了解社會的整體趨勢和態度。媒體也經常報導民調結果,提供公眾對當前議題的見解。
    提供反饋和評估:無論是企業還是政府,都可以透過民調了解其表現、服務或政策的效果,並根據反饋進行改進。
    預測和趨勢分析:民調可以幫助預測某些趨勢或行為的未來發展,如選舉結果、市場需求等。
    教育和提高公眾意識:通過進行和公布民調,可以促使公眾對某一議題或問題有更深入的了解和討論。

    民調可信嗎?
    民意調查的結果數據隨處可見,尤其是政治性民調結果幾乎可說是天天在新聞上放送,對總統的滿意度下降了多少百分比,然而大家又信多少?

    在景美市場的訪問中,我們了解到民眾對民調有一些普遍的觀點。大多數受訪者表示,他們對民調的可信度存有疑慮,主要原因是他們擔心政府可能會在調查中進行操控,以符合特定政治目標。

    受訪者還提到,民意調查的結果通常不會對他們的投票意願產生影響。換句話說,他們的選擇通常受到更多因素的影響,例如候選人的政策立場和政府做事的認真與否,而不是單純依賴民調結果。

    從訪問中我們可以得出的結論是,大多數民眾對民調持謹慎態度,並認為它們對他們的投票決策影響有限。

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